Advances in mechanisms of immunity enhancement induced by thermal ablation therapy in hepatocellular carcinoma

Image-guided tumor thermal ablation plays a key role in the management of hepatocellular carcinoma. Thermal ablation for hepatic tumors not only generates directly killing effect on liver tumors, but also improves the level of anti-tumor immunity of the host. Thermal ablation potentially affects the anti-tumor immunity in the following ways: (1) causing necrosis that is a source for intracellular antigens, and also inducing local inflammation, which further stimulates the antigen-presenting cells; (2) removing the tumor burden; (3) undergoing the process of heat shock in surviving tissue, leading to increase in the expression of heat shock proteins; (4) activating and enhancing tumor-specific T-cell responses. This review discusses these possible mechanisms.     

Construction of a lentiviral RNA interference vector targeting rat myocardin and its effect on vascular smooth muscle cell differentiation

AIM: To construct a lentiviral RNA interference(RNAi)vector targeting rat myocardin mRNA and to investigate its effect on the differentiation of vascular smooth muscle cells(VSMCs).METHODS: Three pairs of dsDNA targeting rat myocardin mRNA were designed, synthesized and cloned into lentiviral vector pGCSIL-GFP to generate pGCSIL-GFP-shMyocd lentvirus. A Flag-tagged myocardin-overexpression vector pEGFP-N1-Myocd was constructed with pEGFP-N1/X124G. After these two vectors were cotransfected into 293T cells, the flag protein was assessed by Western blotting to analyze the knockdown effect of pGCSIL-GFP-shMyocd. The expression of myocardin and SM22α was also detected by RT-PCR and Western blotting after the pGCSIL-GFP-shMyocd viruses were transfected into primary cultured rat aortal VSMCs.RESULTS: The rat myocardin lentviral RNAi vector pGCSIL-GFP-shMyocd and myocardin-overexpression vector pEGFP-N1-Myocd were successfully constructed. After these two kinds of vectors were cotransfected into 293T cells,the No.1 interfering vector displayed the highest inhibitory effect on flag expression.After the No.1 lentvirus at the titer of 1×10¹² TU/L was transfected into VSMCs, the myocardin and SM22α expression was significantly attenuated. CONCLUSION: The lentiviral pGCSIL-GFP-shMyocd RNAi vector is successfully constructed, which is useful for further study regarding the molecular mechanism of the phenotypic switching in VSMCs under special pathological conditions such as atherosclerosis. Inhibition of myocardin expression in VSMCs leads to the decrease in the expression of differentiation marker, and implies a crucial role of myocardin in VSMCs differentiation.     

水牛MD-2cDNA基因的克隆与原核表达

为探讨水牛抵抗革兰阴性菌感染的免疫机制,克隆、表达了水牛髓样分化蛋白-2(bMD-2)基因,并用Western blot进行鉴定.采用RT-PCR方法从水牛外周血白细胞中扩增bMD-2基因,构建pET28a-bMD-2表达载体,转化至大肠埃希菌BL21( DE3)中,IPTG诱导表达,进行SDS-PAGE和Wester...     

乳酸菌对水产养殖动物抗氧化应激与免疫力影响研究进展

简述了在水产养殖动物中使用乳酸菌所具有的抗氧化应激与免疫力影响的研究现状和作用机理,并描述了其应用前景,以期为乳酸菌制剂在水产动物上的应用提供参考。     

温度和烟粉虱寄主植物对海氏桨角蚜小蜂生物学特性的影响

海氏桨角蚜小蜂Eretmocerus hayati(Zolnerowich and Rose)原产于巴基斯坦,是B型烟粉虱Bemisia tabaci (Gennadius)的一种有效天敌.本文报道了不同温度条件(20、22、26、30、34℃,RH65%±5%,光周期14L:10D)和饲养在不同寄主植物--番茄、黄瓜、甘蓝上(26℃,RH65%±5%,光周期14L:10D)烟粉虱对海氏桨角蚜小蜂生物学特性的影响.结果表明,在20℃和30℃恒温条件下,该蜂寄生在番茄上饲养的烟粉虱的发育历期分别为31.7d和12.4d,完成发育的有效积温为247日·度,发育起点温度为11.9℃.在26℃恒温条件下,该蜂寄生番茄、黄瓜和甘蓝上的烟粉虱若虫时,卵至成蜂羽化的发育历期分别为15.7、16.1和15.2d,相应存活率分别为74.6%、72.1%和78.7%,雌蜂寿命分别为7.4、7.6和8.ld,平均繁殖量分别为67.2、62.3和74.7粒卵.本研究结果对海氏桨角蚜小蜂的大量饲养和田间释放具有指导作用.     

基于PC-RELM的养殖水体溶解氧数据流预测模型

养殖水体中溶解氧浓度一直是最重要的水质参数之一。为了精准地对水体溶解氧进行调控，提高养殖生产效率，降低养殖风险，该研究考虑外部天气条件对溶解氧的影响以及溶解氧自身的昼夜变化特征，提出一种基于正则化极限学习机（principal component analysis and clustering method optimized regularized extreme learning machine,PC-RELM）的养殖水体溶解氧数据流预测模型。首先，采用主成分分析法判断影响溶解氧浓度的强重要性因子，降低预测模型的数据维度；其次，利用熵权法计算各时刻点的天气环境指数，并利用快速动态时间规整算法（fast dynamic time warping,FastDTW）完成时间序列数据流在不同天气环境下的相似度度量；然后使用k-means算法对时间序列的相似度进行聚类分簇，并基于分簇结果完成正则化极限学习机预测模型的构建，实现溶解氧浓度的估算。最后将PC-RELM模型应用到无锡南泉试验基地养殖池塘的溶解氧预测调控过程中。试验结果表明：PC-RELM的预测均方根误差值（root mean sq...     

20世纪50年代以来科尔沁沙地景观时空动态变化探析

研究分析了50年来科尔沁流动沙地和半固定沙地景观时空动态变化,并分析了沙地时空动态变化的主要成因。     

安徽江淮丘陵区杂交水稻钾肥效应初报

试验结果表明:杂交水稻每公顷施用K2O135kg时产量最高,达7735kg/hm2,比对照(NPK0)增产12 1%。钾肥经济效益也以每公顷施K2O135kg最高,比对照增收1008元/hm2,产投比为3 3∶1。钾肥施用量在K2O180kg/hm2以下时,土壤中的钾素亏损,不能保持土壤中钾素平衡。     

黑土长期定位试验原状土搬迁对土壤微生物性质的影响

通过测定黑土长期定位试验原状土整体搬迁前后四种处理(CK、NPK、M、MNPK)土壤可培养微生物数量、土壤微生物量含量及土壤酶活性变化,探讨搬迁的特定条件对土壤微生物性质的影响。结果表明,1搬迁前后5年各处理三大类微生物数量、微生物量碳、氮含量和四种土壤酶活性在0~20 cm土层中均高于20~40 cm土层;2每年在两个土层中MNPK处理的三大类微生物数量和微生物量碳、氮含量均最高,CK处理均最低,而每年在两个土层中土壤过氧化氢酶和转化酶活性CK和M处理均明显高于NPK和MNPK处理,而磷酸酶活性CK和M处理则低于NPK和MNPK处理,脲酶活性在4个处理中差异不大;3比较搬迁前后5年间各项微生物指标变化发现,各项指标年际间虽存在变化但程度不大,搬迁后2011年和2012年相对较高,同为小麦茬的搬迁前2010年和搬迁后2013年各项指标较为接近。研究说明,土壤深度和施肥对土壤各项生物指标影响较大,年际间变化也受到轮作方式的影响,搬迁对土壤微生物扰动影响远小于施肥和耕作方式等的影响。     

方正县土壤硒的分布特征及其与土壤性质的关系

方正县是黑龙江省水稻主产区,为研究方正县土壤全硒(Se)的含量、分布特征及其与土壤性质的关系,在哈尔滨市所属方正县境内采集127个0~20 cm耕层土壤样品,分析了方正县土壤全硒含量及其影响因素。结果表明,方正县土壤全硒含量变幅为0.030~0.496 mg kg-1,算术平均值为0.228 mg kg-1略低于全国土壤平均硒含量,总体上处于中等、边缘及缺乏水平。在不同类型土壤中,泥炭土硒含量相对较高(0.267 mg kg-1)而沼泽土硒含量相对较低(0.153 mg kg-1)。不同成土母质中,以坡积物为母质发育的土壤硒含量较高(0.276 mg kg-1)而以冲洪积物为母质发育的土壤硒含量较低(0.207 mg kg-1)。在土地利用方面,稻田土壤全硒含量略高于旱地土壤。相关分析表明:土壤有机碳(SOC)与土壤粒径分布(PSD)是影响研究区表土层土壤硒含量的主要因素。